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. 2019 Apr 15;9:6050. doi: 10.1038/s41598-019-42585-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-immune IgM-binding to recombinant PfEMP1 domains in ELISA. Binding of non-immune IgM to immobilized recombinant proteins representing candidate IgM-binding domains in PF07_0139 (A), HB3VAR40 (B), HB3VAR22 (C), HB3VAR09 (D), IT4VAR46 (E), and IT4VAR67 (F). Means and standard deviations of data from three independent experiments are shown. Dotted lines represent the cut-off above which binding to non-immune IgM was considered as positive. Domain types are color-coded as in Fig. 2B.