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. 2019 Apr 15;9:6050. doi: 10.1038/s41598-019-42585-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-immune IgM-binding and specific PfEMP1 expression on the surface of infected erythrocytes selected by rat anti-PfEMP1 domain-specific IgG. P. falciparum 3D7 (left), HB3 (center), and IT4 (right) with heterogeneous var gene transcription (Fig. S1) after several rounds of selection for IE reactivity with rat anti-PF07_0139, anti-HB3VAR40, and anti-IT4VAR67, respectively, and labeled with the antibody used for selection (A) or non-immune IgM (B). Background labeling indicated in black. Correlation (r_s) of non-immune IgM-binding and labeling by PfEMP1-specific IgG for all selected parasites expressed as percentage of IEs (C).