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. 2019 Apr 15;9:6025. doi: 10.1038/s41598-019-42487-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of flow cytometric cell counts of aplastidic protists counted live and with two cryoprotectants. Triplicate samples of coastal Maine water were run live and stained with either Lysotracker (LT) or SYBR green. Replicate samples were stored with the cryoprotectants glycine betaine (GBe) or glycerol – TE (Gly-TE) at −80 °C and then stained with SYBR green before enumeration. Each replicate was run with the flow cytometer triggered by green fluorescence (FL1) or by side scatter (SSC). Errors bars show standard deviations of triplicate samples.