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. 2019 Apr 15;9:6078. doi: 10.1038/s41598-019-42523-0

Figure 5.

Figure 5

Agarose gel and densitometry analysis of the RT-PCR products (in duplicates) showed exon-23 and exon-22/23 dual skipping in mdx mouse myotubes in vitro. Concentrations of AOs used include 2.5 nM, 5 nM, and 12.5 nM. (A) Fully modified 2′-OMe-PS control AO; (B) 2′-OMe modified 2′-F-PS AO chimeras including 2′-OMe/2′-F-PS gapmer, 2′-OMe/2′-F-PS mixmer 1, and 2′-OMe/2′-F-PS mixmer 2; (C) LNA modified 2′-F-PS AO chimeras including LNA/2′-F-PS gapmer, LNA/2′-F-PS mixmer 1, and LNA/2′-F-PS mixmer 2. The gel images and their corresponding densitometry data of each repetition are shown in Figs S2 and S4 (Supplementary Information).