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. 2019 Apr 15;9:6089. doi: 10.1038/s41598-019-42423-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of Minimal Inhibitory Concentration (MIC) of 16 and 32 mg/L to detect the presence of specific macrolide-resistance mechanisms. R: Resistance; R_PAβN: resistance in presence of PAβN. (a) Overall. (b) Isolates in which no sought mechanisms of resistance was found. (c) Isolates carrying the mph(A) gene alone or with a target mutation. (d) Isolates carrying the mph(A) gene together other MRG. (e) Isolates carrying a MRG different that mph(A). (f) Isolates carrying only L4 and/or L22 amino acid changes. ^*The single isolate (isolate 3491) which remains resistant after PAβN addition possesses an unidentified MRG.