Fig. 7.

Mechanical maturation of the centromere correlates with ploidy in cancer cells. a Probability density functions for individual observations of total chromosome number in HT-1080, HeLa, U-87, and U2OS cells. Sample sizes (n) reflect the number of cells per sample. Dotted lines and data labels indicate the median chromosome number in each cell line. b, c Relationship between percent change in mean centromere stiffness and ploidy for early- to late-prometaphase (L) and late-prometaphase to metaphase (M) across the four cancer cell lines, with data from non-cancerous RPE-1 cells shown for comparison. X-axis error bars represent IQR; Y-axis error bars represent standard error. All n-values listed are chromosome numbers. See also Supplementary Figs. 2–4 and 6–7