Fig. 3. miR-142-3p affects the expression of anti-inflammatory cytokines and pro-inflammatory cytokines in in vitro induced Tregs (iTregs) (n = 3).

iTregs were expanded in vitro and were then untreated or were incubated with an inhibitor or mimic. a Representative example of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-10 (IL-10), and tumor growth factor-β (TGF-β) histograms for iTregs treated or not treated with the inhibitor or mimic (gated on CD4+CD127− cells). Summary of overall. b, and c Expression of TNF-α and IFN-γ was decreased in iTregs treated with the inhibitor, compared with the control. d, e Levels of IL-10 and TGF-β were decreased in iTregs treated with the mimic compared with the control. Results are presented as mean ± standard error of the mean values. **P < 0.01; ns not significant