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. 2019 Apr 15;9:6080. doi: 10.1038/s41598-019-42570-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clustering analysis of genes differentially expressed between developmental stages. A total of 9612 genes are clustered into 10 (C1 to C10) based on expression dynamics by K-means clustering. The upper-left alphanumeric values designate the number of the cluster. Sex-biased and dauer-biased clusters were indicated by stars and triangles, respectively. The upper-right values in each cluster shows cluster size (number of genes). Black lines indicate representative transcriptional expressions.