Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 15;9:6029. doi: 10.1038/s41598-019-42413-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Selective labelling the right PN neurons and optogenetic stimulation of mossy fibers in the left MCP. (a) The rats were stereotactically injected with pAAV 2/9-hSyn-ChR2-mCherry or pAAV 2/9-hSyn-mCherry targeting the right PN. (b) Example of ChR2-mCherry expression in the right PN. (c) Representative images showing cell-specific ChR2-mCherry expression (red) in neurons (green) of the PN. (d) Statistics of expression in the neurons (468 cells, from 4 mice). (e) In vivo right PN “optrode” recording setup. (f,g) Multi-unit activity in the right PN from a rat injected with pAAV2/9-hSyn-ChR2-mCherry in response to trains of 7 light pulses (470 nm, 10 mW/mm², 20 Hz, 15 ms pulse duration). Blue bars represent light on. (h) Schematic illustration of in vivo optical stimulation and LFP recording in the left MCP. (i) Example of ChR2-mCherry expression in the left MCP. White dashed line: optrode position. (j,k) Trains of 7 light pulses (470 nm, 25 mW/mm², 20 Hz, 15 ms pulse duration) also evoked robust LFP responses in the left MCP of a wake behaving rats. Note that the graph. (j) Illustrates an example of the mean value of 100 light-induced LFPs. Data are represented as mean ± s.e.m.