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. 2019 Apr 15;10:1751. doi: 10.1038/s41467-019-09756-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Uncoupling of PRC1 and RNF168 ubiquitination. a Top view on the nucleosome highlighting the 180° rotated position of UbcH5c in the RNF168 (left) compared to RING1B nucleosome complex (right). Color coding indicated in the Figure, position of the target lysines is indicated with the blue sphere. b Side-by-side comparison of the RING domain positions on the acidic patch, RNF168 (right) and RING1B (left). Secondary structure elements are labeled to highlight the approximate 180° turn of the RING domain, also indicated by the arrow. The H2B E110 (E113 in humans) side chain is close to the E3 RING surface for RNF168 but far from the surface of RING1B. Selected side chains are shown as sticks. c Nucleosome ubiquitination by either RNF168^RING or RING1B/BMI1 on wild-type (WT) or H2B E110A nucleosomes. Fluorescent TAMRA-labeled ubiquitin was used to detect ubiquitinated species. Samples were resolved using SDS-PAGE. For all samples time points were taken at 2, 6, 18, and 40 min of incubation of the reaction mixture. Molecular size of mono- and di-ubiquitinated H2A is indicated. Source data are provided as a Source Data file

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