Fig. 6.

Comparison of acidic patch binding mode of RNF168 and RING1B. a Superposition of RNF168^RING on the RING1B structure results in severe clashes of the Arg-rich helix with the H2B αC helix. b Molecular surface of the bound states of RNF168 and RING1B RING domains with color coded electrostatic potential, calculated for the solvent accessible surface (color scale shown in panel c). Both RING domains are shown looking down on their nucleosome binding surface in an aligned orientation. Location of the E2 interface is indicated with a solid line. c Comparison of the acidic patch binding modes of RNF168^RING and RING1B^RING, both shown as molecular surface with color coded electrostatic potential, calculated for the solvent accessible surface, and aligned on the nucleosome. The yellow and orange dashed boxes delineate the main interaction sites of RNF168 and RING1B. Selected residues are labeled