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. 2019 Apr 15;4:15. doi: 10.1038/s41541-019-0109-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of terminal sera from NIPARAB immunized mice. a, b Average EC50 values (bars) were derived from ELISA curves measuring either total NiV G IgG (left panel) or RABV G (middle panel) from individual mice (open circles) from each vaccination group. Statistical significance was performed using 2-way ANOVA followed by uncorrected Fisher’s LSD to compare immunization groups (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). c IgG1 and IgG2 isotype responses in final sera (day 45) from live or inactivated NIPARAB-immunized mice were assessed by ELISA at day 45 and average EC50 values (bars) were determined based on individual ELISA curves (open circles). Statistical significance was performed using 3-way ANOVA followed by uncorrected Fisher’s LSD test to compare immunization groups (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Error bars represent standard deviation from the mean of three replicate values