Fig. 4.

Staggered commitment of splenic white pulp organizer cells along the developing arterial tree. a, b Ccl19-iEYFP mice were treated with doxycycline from E19.5 (a, c) or P7 (b, d). Distribution and phenotype of EYFP⁺ cells in the white pulp of 6-week-old mice were determined by confocal microscopy using the indicated antibodies. Boxes show region of higher magnification in right images, arrows indicate EYFP⁺MAdCAM-1⁺ MRC c, d, arrowheads indicate EYFP⁺PDPN⁺ TRC (c, d), asterisks indicate EYFP⁺CD21/35⁺ FDC (c, d) (scale bars = 500/20 μm). e Determination of EYFP⁺ cell distances from the central artery at the hilum. Cross-sections from Ccl19-iEYFP spleens from 6-week-old mice were stained with antibody against TER119 and EYFP expression was pseudocolor-coded (lower image) according to concentric distance from the hilum (0–2400 μm). Scale bar = 250 μm. f Frequency of EYFP⁺ cells in white pulp areas of indicated concentric distances from the central artery (n = 5–7 mice per group from two independent experiments, mean ± SEM). Statistical analysis was performed using a Kolmogorov–Smirnov test. Source data are provided as Source Data file