Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 15;9:6056. doi: 10.1038/s41598-019-42403-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

RelA dependent persister cell formation induced by limitation of tRNA charging. Stationary phase MG1655valS^ts cells were diluted 1:1000 and grown under semi-permissive conditions (at 36.6 °C) until OD₆₀₀ ~ 0.1 in a temperature-controlled microtiter plate reader, in supplemented M9 medium. MG1655, MG1655ΔrelA, and MG1655ΔrelAvalS^ts cells were used as controls. (A) Cells were treated with 150 μg/ml ampicillin, and the optical density of the cultures was recorded over time. The continuous decrease of OD₆₀₀ over the 16 h incubation with ampicillin indicates that no resistant mutants appeared in the samples. (B) Colony forming units were determined before and at different time points during treatment; the ratios of survivors after 6 h and 16 h of treatment were plotted.