Fig. 4.
Effect of UCAE on [Ca2+]i in RBL-2H3 cells. Anti-DNP IgE-sensitized cells were incubated with Fluo-3 AM for 1 h and then treated with UCAE (3.2 mg/mL) or with 5 mM NaPB. The cells were then stimulated with antigen and the fluorescence intensity was measured. Open triangles indicate NaPB-treated cells not stimulated with antigen; open circles indicate NaPB-treated cells stimulated with antigen as a control; closed circles indicate UCAE-treated cells stimulated with antigen. Data are represented as the mean ± SD of triplicate of wells. Dunnett’s test was used to assess the statistical significance of the deference (**P < 0.01) against control