Fig. 5.

Effect of UCAE on the signaling pathways involved in degranulation of RBL-2H3 cells. Anti-DNP IgE-sensitized cells were treated with UCAE (3.2 mg/mL) or with 5 mM NaPB and stimulated with antigen for 5 min. Each cell lysate was then used for immunoblot analysis. a p-Lyn, p-Syk, p-PI3K, p-Btk, p-PLCγ1, p-PLCγ2, and p-Akt indicate phosphorylated Lyn, phosphorylated Syk, phosphorylated PI3K, phosphorylated Btk, phosphorylated PLCγ1, phosphorylated PLCγ2, and phosphorylated Akt, respectively. A representative blot from three independent experiments is shown. b The ratio of phosphorylation in each cell lysate relative to that in the control cells. Gray bars indicate NaPB-treated cells not stimulated with antigen; open bars indicate NaPB-treated cells stimulated with antigen as a control; closed bars indicate UCAE-treated cells stimulated with antigen. Data are represented as the mean ± SD of three independent experiments. Dunnett’s test was used to assess the statistical significance of the deference (*P < 0.05; **P < 0.01) against control