Fig. 6. GSNO induces A549 apoptosis via p53-dependent p21 induction.

a The interaction between SIRT1 and p53 (**P < 0.01, using unpaired two-tailed Student’s t-test). b Expression of p53 acetylation and p21 after GSNO treatment for 24 h (*P < 0.05, **P < 0.01, using one-way ANOVA followed by Bonferroni’s multiple comparisons test). c The effect of N6022 (0.5 μM) on the expression of p53 acetylation and p21 after GSNO treatment (**P < 0.01; ns, not significant, using one-way ANOVA followed by Bonferroni’s multiple comparisons test). d, e Overexpression Prdx2 attenuated the increased expression of p53 acetylation and p21 induced by GSNO in A549 cells (**P < 0.01, using two-way ANOVA followed by Bonferroni’s multiple comparisons test). f Expression of p53 acetylation and p21 after H₂O₂ (50 μM) treatment (**P < 0.01, using unpaired two-tailed Student’s t-test). g The effect of GSNO on the expression of p53 acetylation and p21 with AICAR (1 mM) or Compound C (20 μM) (*P < 0.05, using one-way ANOVA followed by Bonferroni’s multiple comparisons test). h Schematic representation of the mechanism by which GSNO promotes apoptosis in A549 cells. The data are expressed as the mean ± SD of three independent experiments