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. 2019 Apr 9;10:678. doi: 10.3389/fimmu.2019.00678

Figure 2.

Figure 2

Analysis of iBET151-modulated proteins and genes expressed by in vitro-cultured human ILC2s. (A) Method for stimulating hILC2s in vitro with IL-33 and iBET151. Intracellular flow cytometry detection of (B), IL-13 and (C), IL-5 protein expression in hILC2 cultures in the absence or presence of iBET151. (D) Cytokine analysis of culture supernatant taken from in vitro expanded hILC2s following IL-33 restimulation for 24 h. (E) Volcano plot of genes differentially expressed between hILC2s stimulated with IL-33 alone or with IL-33 and 125 nM iBET151 (>1.7 fold change). (F) Gene ontology summary of immune system process genes lowered by iBET151. (G) Heat map of immune system process genes from gene ontology analysis in (F). Data include three individual donors, processed independently before restimulation and library preparation. (H) Venn diagram of common and distinct genes from Figure 1C and Figure 2F.