Skip to main content
. 2019 Apr 9;10:341. doi: 10.3389/fneur.2019.00341

Figure 2.

Figure 2

Effects of administration of MR16-1 on the descending pain inhibitory system in pre-onset EAE mice. (A–D) Spinal 5-HT and 5-HIAA was measured in control and pre-onset EAE mice (clinical score 0) on Day 20 after immunization. Levels of spinal 5-HT were decreased in EAE mice (A). Levels of spinal 5-HIAA, the major metabolite of 5-HT, were increased in EAE mice (B). 5-HIAA/5-HT ratio was increased in EAE mice (C). *p < 0.05 vs. Control + Vehicle by Steel-Dwass test (n = 4–8 per group). (D) Representative images of BOLD responses in the PAG. Blue area indicates PAG. Red and yellow areas show significant changes of BOLD signals. (E) The changes of BOLD signals in PAG were significantly decreased in pre-onset EAE mice. (F) There is a negative correlation between the changes of BOLD signal intensity in PAG and MGS score. Black bars indicate the mean value of each measurement. *p < 0.05 vs. Control + Vehicle by Tukey's multiple comparison test (n = 5–6 per group).