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. 2019 Apr 9;10:731. doi: 10.3389/fimmu.2019.00731

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Copyright © 2019 Xie, Wang, Cheng, Zhao, Liu, Zhu, Chen, Jia, Yang, Wu, Zhang, Liu, Yu, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SOCS3 inhibits the expression of IFNα. pCAGGS-SOCS3-His and pCAGGS-IFNα/γ-FLAG were co-transfected into CEFs, which were harvested 36 and 48 h after transfection. (A) Western blot analysis of SOCS3 expression. (B) Quantitative PCR analysis of SOCS3 mRNA levels. (C) Western blot analysis of IFNα/γ expression. (D) Quantitative PCR analysis of IFNα/γ mRNA levels. Differences between two groups were analyzed using Student's t-test and considered significant at ^*p < 0.05 and ^**p < 0.01.