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. 2019 Apr 9;10:731. doi: 10.3389/fimmu.2019.00731

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Copyright © 2019 Xie, Wang, Cheng, Zhao, Liu, Zhu, Chen, Jia, Yang, Wu, Zhang, Liu, Yu, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of SOCS3 and IFNα. (A) Localization of IFNα (green) in CEFs. DAPI staining shows the nucleus in blue. (B) Localization of SOCS3 (red) in CEFs. DAPI staining shows the nucleus in blue. (C) Co-localization of IFNα (green) and SOCS3 (red) in CEFs. DAPI staining shows the nucleus in blue. (D) CEFs were transfected with pCAGGS-IFNα-Flag and pCAGGS-SOCS3-His for 48 h before the coimmunoprecipitation and immunoblot analysis with the indicated antibodies.