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. 2019 Apr 9;10:432. doi: 10.3389/fpls.2019.00432

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Copyright © 2019 Dolgikh, Kirienko, Tikhonovich, Foo and Dolgikh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast two-hybrid assay (GAL4 transcription factor-based) for DELLA1 protein and NSP2 and IPD3/CYCLOPS transcription factors and their truncated variants corresponding to those in E69 (nsp2/sym7-1), RisNOD14 (nsp2/sym7-2) and SGEFix^-–5 (ipd3/cyclops/sym33-2). AD – activation domain of GAL4, DBD – DNA binding domain of GAL4. The pairs of vectors pEXP32/Krev1 and pEXP22/RalGDS-wild type, pEXP22/RalGDS-m1 and pEXP22/RalGDS-m2 were used for strong, weak and not detectable interactions. Yeast growth was tested on SC medium –LTH + 50 mM 3 AT and SC – LTU.