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. 2019 Apr 9;10:432. doi: 10.3389/fpls.2019.00432

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Copyright © 2019 Dolgikh, Kirienko, Tikhonovich, Foo and Dolgikh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of PsGA20ox, PsKNOX, and PsBELL genes in wild type cv. SGE and SGEFix^-–2 (sym33–3), SGEFix^-–5 (sym33–2) mutants 2 weeks after inoculation. Transcript levels of genes were normalized against ubiquitin gene. For each gene, the transcript level in non-inoculated roots of wild type or mutants was set to 1 (control), and the level in nodules of wild type or mutants was calculated relative to the control values. Data are averages ± SEM (n = 6–8 plants of wild type or mutant combined from two independent experiments). Values with different letters are significantly different (P < 0.05) as analyzed by one-way ANOVA and the Tukey’s test as post-hoc analysis.