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. 2019 Apr 9;10:444. doi: 10.3389/fpls.2019.00444

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Copyright © 2019 Lin and Sauter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of auxin transport inhibition on programmed cell death (PCD) and AR emergence. (A) Cell death of epidermal cells above AR primordia, analyzed with Evans Blue at the third node of stem sections kept in the dark for 6 h, 14 h or 24 h with or without 5 μM NPA. (B) Percentage of penetrated ARs. Bars in A, B indicate means ( ± SE) of ARs from 9 stems per treatment measured in 3 independent experiments. Different letters indicate statistically significant differences (P < 0.05; ANOVA with Tukey test). (C) Auxin activity detected with DR5:VENUS in AR primordia with or without 5 μM NPA for 1 day; Bar=25 μm.