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. 2019 Apr 9;10:453. doi: 10.3389/fmicb.2019.00453

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Copyright © 2019 Garel, Bonin, Martini, Guasco, Roumagnac, Bhairy, Armougom and Tamburini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Cross section of the Titanium TA6V 500 mL High Pressure Bottle (HPBs). (B) Schematic drawing of the front and back views of the High-Pressure Sample Unit (HPSU). 1. Top end-cap devoted to oxygen measurement; 2. 1/8″ (3.2 mm O.D.) Inlet flowthrough; 3. Sapphire window; 4. Main core; 5. PEEK floating-piston with lip seal; 6. Bottom end-cap. In the part, above floating-piston seawater sample is in contact with sapphire window (see details in the text). The part below the floating-piston contains sterile milliQ water which serve as hydraulic brakes when passing through the thin catheter into the exhaust tank (7); 8. Polypropylene main frame; 9. Push rod for attachment to a rosette system. When the (10) inlet-valve is opened by magnetically activated lanyard release, the seawater enters the hydraulic circuit through a 1/8″ (3.2 mm O.D.) stainless-steel tube (11), via one check valve (12), to fill in the HPB (13), the pressure accumulator (14), and the aero-hydraulic pressure sensor (15).