Figure 4.

Timeline of the incubation experiment. Deep-sea samples were recovered at 3,000 m-depth and incubated at in situ pressure conditions (HP) or after decompression at atmospheric pressure conditions (DEC). Samples were firstly incubated at in situ temperature over 7 days. Then, the second incubation was made by transferring, in equi-pressure mode for each condition, 50 mL of the first incubated sample into a new high-pressure bottle (HPB) containing 450 mL of 0.2 μm-filtrated and sterilized seawater, sampled at the same depth. The remaining 450 mL of the first-incubated HPBs were then quickly sampled and conditioned for sequencing analysis (T₀ HP and T₀ DEC). At the end of the 2nd incubation, a third incubation was made according to the same protocol. At the end of each incubation the remaining 450 mL was conditioned for sequencing analysis and named T₁ HP/T₁ DEC and T₂ HP/ T₂ DEC, respectively. Dissolved-oxygen concentration was used to determine the incubation time. Graph plots represent dissolved-oxygen concentration (expressed in percent of initial dissolved-oxygen concentration) against time (h).