Table 1.
Primers used for the amplification and full gene sequencing.
| Gene | Purpose | Name of primer | Forward primer sequence (5′ –3′) | Name of primer | Reverse primer sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|---|---|---|---|
| gyrA | PCR | gyrA F | CGCCAATAAACGCCAAGACC | gyrA R | AGCGGTAAATGACGTAGCCC | 2769 |
| Sequencing | F-1 | GGTGAACCTCAACGACGGC | R-1 | ACTGCGATACCGGAAGAACC | ||
| Sequencing | F-2 | CTATGACGGTACGGAAAAAAT | R-2 | CGGTGGCGCACGAACGCTGAAATG | ||
| Sequencing | F-3 | GACCCAGCTACAGGTTTCCTTC | R-3 | CTGGCTAATCAGATCTTCGATATT | ||
| Sequencing | F-4 | ATCCGCGAAGAGATGGAGTTA | R-4 | GACGGCGTCTTCTTTGAAGC | ||
| gyrB | PCR | gyrB F | TAGCGCTGAACACGTTATAGAC | gyrB R | GCCACTCAGCGCTTTAGAGAT | 2584 |
| Sequencing | F-1 | CAGCGAGATGGCAAAATTCAC | R-1 | CGCAATCAGACCTTCACGGG | ||
| Sequencing | F-2 | TGGACAAAGAAGGCTACAGCA | R-2 | CTTGTCCGGGTTGTACTCGT | ||
| Sequencing | F-3 | TCGACAAGATGCTTTCCTCCC | R-3 | CATCGAACTTCCACTGACTGC | ||
| Sequencing | F-4 | GCTGGGTGAATGCGCTG | R-4 | GCCAGAAACGTACCATCGTG | ||
| parC | PCR | parC F | CCACCTGTCTCACGGTTTGT | parC R | GGCCGGATAAGACGCTACTG | 2419 |
| Sequencing | F-1 | AATACGCCGAGCTGCTGTTA | R-1 | GTGGTGGCGAACAGATGGTT | ||
| Sequencing | F-2 | ACCCGACGCGTTTAGTGATT | R-2 | GCTTTAGCTTCTTCGCGCTC | ||
| Sequencing | F-3 | GTCCGAACGCAAAATGAATACC | R-3 | TCTTCAATCACCAGCGGCG | ||
| Sequencing | F-4 | GCTACGGCTTCGTTTGTACG | R-4 | CGCCGGTAACATTTTCGGTT | ||
| parE | PCR | parE F | GCATTCACGGGCTTCGAAGATT | parE R | ATGCGCAAGTGTCGCCATCA | 2060 |
| Sequencing | F-1 | CGCGACGGTCAGGTCTATAA | R-1 | CGACAGACGCTCTTTGGTCT | ||
| Sequencing | F-2 | ATACCGCAATATTCTGCCGC | R-2 | GACTCAGATCGTCGCTGTCC | ||
| Sequencing | F-3 | CTTCCGATGAAGTGCTGGC | R-3 | AATGGACGCTGGTTCCAGTA | ||
| acrA | PCR | acrA F | AAACGCAGGGCCACATCCAG | acrA R | TGATGGCGATCACCCACGCA | 1230 |
| Sequencing | F-1 | GATTGATCCTGCGACCTACCA | R-1 | GGCGCGCAAAGTAATAGACC | ||
| Sequencing | F-2 | AAACAGGAAAACGGCAAAGCG | R-2 | TTCACCGTCAGTTCAGCGAT | ||
| acrB | PCR | acrB F | GCGCACAGGTTAAAGTACA | acrB R | AATAATCAATCTAACAATAAGCGT | 3295 |
| Sequencing | F-1 | CGTGAGCGTTGAGAAGTCC | R-1 | GGCGTGGTGTCATACGGG | ||
| Sequencing | F-2 | TTGGCATCAAACTGGCTACC | R-2 | CGTCAGGATCAGCGCGAC | ||
| Sequencing | F-3 | CAACCGGGGCAATTTATCGT | R-3 | ATTTGTGAAAACGCTGCGG | ||
| Sequencing | F-4 | CCGGTATTGCATTCGTGTCG | R-4 | TTATAGCGTTCCAGACGCGG | ||
| Sequencing | F-5 | AACGACTGGTACGTTCGTGG | R-5 | ACCCAATGGTTGTGAGCAGG | ||
| Sequencing | F-6 | CTATCCCGTTCTCCGTAATG | R-6 | AGAGGTACGGCTGATCGGA | ||
| tolC | PCR | tolC F | CTGCTGTTTTATCACCCTTGTGG | tolC R | TCAGGCGCGGCTTTATGAC | 1692 |
| Sequencing | F-1 | CTTTCCTATACCCAGGCGCA | R-1 | CTTTCCAGCTGTTCGCTTGC | ||
| Sequencing | F-2 | TGGGATGGTTAACTCGCAGG | R-2 | TTCGCGGTATTGAGGATCAGC | ||
| ompC | PCR | ompC F | CCGTTGATTTTAAAAGTTTCGT | ompC R | GGACGCAAGCGTATATCAAA | 1301 |
| Sequencing | F-1 | GATGGCGACCAGACCTACAT | R-1 | TACTGCGCTGCCAGATAGAT | ||
| Sequencing | F-2 | GGCGCTATCACCACGTCTAAA | R-2 | TTCAGTCTGGTTGCCCTGAA | ||
| ompF | PCR | ompF F | ACCAAATCTTTATCTTTGTAGCA | ompF R | CCTGTTTTTGAAAGACGCAC | 1245 |
| Sequencing | F-1 | CTAAAGCAGACCGCGCTGA | R-1 | AACGGCCCACGATTCTGC | ||
| Sequencing | F-2 | AGCGTACAGCAACAGCAAGC | R-2 | CGACCATAATCGATTGAACCC | ||
| ramRA | PCR | ramRA F | CATCGTACTGTGGGCCGAA | ramRA R | TGGTTTCTGTTGCTCGGCG | |
| Sequencing | F-1 | ACGAGTCATCATTTTGGCATC | R-1 | AAGCATAGTAATAACCACACAAA | ||
| Sequencing | F-2 | TTGACGGCGTATCTTTGCTTT | R-2 | GCGACCAAAGATGAGCTGATTA | ||
| rpoE | PCR | rpoE F | AACATGGTTGCGGCAGATTAG | rpoE R | ACGTTTCGCCATCCATCAAAG | 1014 |
| Sequencing | F-1 | GAGCGAGCAGTTAACGGACCA | R-1 | GGAAACCAGACTCGCCACT | ||
| cpxR | PCR | cpxR F | TAACTTTGCGCATCGCTTGC | cpxR R | ATCGAGCTTGGGCAACATCA | 917 |
| Sequencing | F-1 | CGCGGACGACTATTTACCCA | R-1 | GGCGCAAAATAGCCCTGATG | ||
| lpxA | PCR | lpxA F | CGTGGCCTGACCCGCTTTA | lpxA R | CACGAATTAAGCCTGCGCCA | 1128 |
| Sequencing | F-1 | GTCGCACATGATTGTACGGT | R-1 | TCATCGACTGATACGTGCCCC |