. 2019 Apr 9;10:152. doi: 10.3389/fphys.2019.00152

Table 7.

Nutritional reserve amounts of proteins, soluble carbohydrates, glycogen and total lipids in A. aegypti larvae raised in different microorganism-based diets, and their tissues.

	Tetramin^®	S. cerevisiae	Pseudozyma sp.	A. platensis	Chlorella sp.	E. coli	S. aureus	S. marcescens	Bacillus sp.	Asaia sp.	O. intermedium
Protein (mg)
Larvae	250 ± 30	190 ± 10^∗	171 ± 6^∗∗	60 ± 7^∗∗∗∗	50 ± 10^∗∗∗	117 ± 6^∗∗∗∗	64 ± 0.7^∗∗∗∗	48 ± 1^∗∗∗∗	52 ± 0.8^∗∗∗∗	89 ± 3^∗∗∗∗	55 ± 0.7^∗∗∗∗
Gut	77 ± 1	44 ± 2^∗∗∗	48 ± 1^∗∗∗	4 ± 0.4^∗∗∗∗	6 ± 0.4^∗∗∗∗	14 ± 2^∗∗∗∗	9 ± 0.3^∗∗∗∗	6 ± 0.3^∗∗∗∗	3 ± 0.5^∗∗∗∗	10 ± 1^∗∗∗∗	4 ± 0.2^∗∗∗∗
Carcass	169 ± 2	133 ± 5	116 ± 9^∗∗	50 ± 10^∗∗∗∗	43 ± 4^∗	89 ± 5^∗∗∗∗	50 ± 2^∗∗∗∗	39 ± 1^∗∗∗∗	46 ± 1^∗∗∗∗	76 ± 3^∗∗∗∗	51 ± 2^∗∗∗∗
Carbohydrate (mg)
Larvae	125 ± 3	103 ± 1	82 ± 0.8	10 ± 0.3^∗∗∗∗	9.2 ± 0.4^∗∗∗∗	21 ± 3^∗∗∗∗	13 ± 1^∗∗∗∗	5 ± 0.3^∗∗∗∗	5 ± 0.4^∗∗∗∗	16 ± 0.3^∗∗∗∗	11 ± 0.1^∗∗∗∗
Gut	23 ± 2	17 ± 0.8^∗∗∗	16 ± 0.6^∗∗∗∗	2 ± 0.1^∗∗∗∗	2 ± 0.1^∗∗∗∗	1.4 ± 0.1^∗∗∗∗	3 ± 0.1^∗∗∗∗	0.7 ± 0.1^∗∗∗∗	0.8 ± 0.1^∗∗∗∗	1 ± 0.1^∗∗∗∗	2 ± 0.1^∗∗∗∗
Carcass	82 ± 3	77 ± 1^∗∗	61 ± 0.4^∗∗∗	6.9 ± 0.3^∗∗∗∗	6.7 ± 0.3^∗∗∗∗	13 ± 0.6^∗∗∗∗	7 ± 0.4^∗∗∗∗	4 ± 0.2^∗∗∗∗	4 ± 0.2^∗∗∗∗	14 ± 0.4^∗∗∗∗	8 ± 0.1^∗∗∗∗
Glycogen (mg)
Larvae	20 ± 0.5	12 ± 0.8^∗∗∗	10 ± 0.3^∗∗∗	2 ± 0.1^∗∗∗∗	3 ± 0.2^∗∗∗∗	4 ± 0.9^∗∗∗∗	3 ± 0.4^∗∗∗∗	1 ± 0.5^∗∗∗∗	1 ± 0.3^∗∗∗∗	3 ± 0.4^∗∗∗∗	2 ± 0.1^∗∗∗∗
Gut	5 ± 0.2	3 ± 0.5	3 ± 0.5	0.8 ± 0.05^∗	0,9 ± 0.1^∗	0.3 ± 0.04^∗	0.3 ± 0.1^∗	0.2 ± 0.1^∗	0.1 ± 0.04^∗∗	0.2 ± 0.02^∗	0.3 ± 0.03^∗∗
Carcass	11 ± 0.5	6 ± 0.3^∗∗	6 ± 0.5^∗∗	1 ± 0.1^∗∗	2 ± 0.2^∗∗	3 ± 0.3^∗∗∗	3 ± 0.2^∗∗∗	1 ± 0.1^∗∗∗	1 ± 0.1^∗∗∗	2 ± 0.3^∗∗∗	1 ± 0.1^∗∗∗
Lipid (mg)
Larvae	111 ± 1	71 ± 4^∗	79 ± 5^∗∗	19 ± 1^∗∗	16 ± 0.7^∗∗∗	36 ± 4^∗	23 ± 4^∗∗∗∗	7 ± 0.4^∗∗∗∗	6 ± 0.6^∗∗∗∗	27 ± 0.8^∗∗	17 ± 0.4^∗∗∗∗
Gut	32 ± 3	17 ± 1^∗∗	22 ± 0.6^∗∗	3 ± 0.5^∗∗∗∗	3 ± 0.4^∗∗∗∗	1 ± 0.4^∗∗∗∗	1 ± 0.1^∗∗∗∗	0.2 ± 0.04^∗∗∗∗	0.3 ± 0.03^∗∗∗∗	2 ± 1^∗∗∗∗	1 ± 0.1^∗∗∗∗
Carcass	70 ± 4	53 ± 1^∗∗	51 ± 2^∗∗	14 ± 1^∗∗∗	12 ± 0.9^∗∗∗	28 ± 0.2^∗∗∗	17 ± 2^∗∗∗	6 ± 0.2^∗∗∗∗	5 ± 0.2 ^∗∗∗∗	22 ± 0.7^∗∗∗	15 ± 0.8^∗∗∗
Fresh Weight (mg)
Larvae	7 ± 0.0004	6 ± 0.001	6 ± 0.0004	3 ± 0.0003^∗	2 ± 0.0003^∗	3 ± 0.0003^∗	3 ± 0.0003^∗	3 ± 0.001^∗	3 ± 0.001^∗∗	3 ± 0.0003^∗	3 ± 0.0003^∗∗
Gut	3 ± 0.001	3 ± 0.0002	3 ± 0.0003	1 ± 0.0003 ^∗∗	1 ± 0.0003^∗∗	1 ± 0.0002^∗∗	1 ± 0.0002^∗∗	1 ± 0.0001^∗∗∗	1 ± 0.0002^∗∗∗	1 ± 0.0001^∗∗	1 ± 0.0003^∗∗
Carcass	5 ± 0.002	4 ± 0.0001	4 ± 0.0003	2 ± 0.001^∗	2 ± 0.0003^∗	2 ± 0.0003^∗∗∗	2 ± 0.0003^∗∗∗	2 ± 0.001^∗∗∗	3 ± 0.001^∗∗∗	2 ± 0.0003^∗	2 ± 0.0001^∗∗

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005, ^∗∗∗∗p < 0.001.