Table 1.

Primer sequences for amplification

Amplicon	Forward primer	Reverse primer
Exon1 & 2	CGCTCTCCCTCCATGTTAGC	TTCCCCCTACCCCTTTGTGG
Exon3	GAAATCATTGGCACTGCCTG	AGCTGTTTAAGACCATGAGCC
Exon4	AGCTTTGAGAGGCTGTGGTC	TCTCTGGTGGTGGAGAGTCC
Exon5	ATCTTATGAGCTTGGCAGGG	GAAAAGGTTACAAACATTCCCC
Exon6	GTCTGGGAAGGTGTGATCGG	TGCCAGATATCACTGGGGTC
Exon7	GGGAAAGGGTTTAAGTCCCC	TGGAGTAATGATGGAACAGGG
Exon8	CCACCAATCCTCTCTATCTTGG	GCTTACTTCCTTTCTTCTGAGCG
Exon9	CACCCCAACTTCTCTCATGC	TAGCAAAGATGCCCTGTTCC
Exon10	CACTGGGCAGTGTTTGAATG	AGGTAGATCTTGGCGGATTC
Exon11 & MIR32–5pa bs	TTCTCTCTGGCATCGTGTGC	ACGCCTATTTTCTCTTGCATCT
MIR198 & MIR27a bs	GAAAGACACAGGACTAACTGT	CCACTATCTGCCAGAGGAGCA
MIR206 bs	CACTGGATCTTAACAGATGC	CTTCAAACTCCACTTGTTCAGT

Sequence of the forward and reverse primers used to analyze the coding region of FSTL1 locus (NM_007085.5); bs: binding site.