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. 2019 Apr 9;10:433. doi: 10.3389/fpls.2019.00433

FIGURE 5.

FIGURE 5

Silence of MoAP1 by asiRNAs resulted lower virulence of M. oryzae. (A) Disease phenotypes of TP309 at 7 days post-inoculation (dpi) by asiRNA-treated M. oryzae strains Guy11. Rice cultivar TP309 was punch-inoculated with Guy11 (1 × 105 spores/ml) cultured on media containing the indicated asiRNAs targeting MoAP1. (B) Quantitative analysis on lesion area at 7 dpi. The length and width of the symptom were measured, and the product of the length and width were designed as the area of the symptom. Black lines in lesion (A) were used to represent length and width. Values were means ± standard deviations (SD) of 15 disease spots. Asterisks (∗∗) above the bars indicate significant differences at P < 0.01 as determined by student’s t-test analysis. (C) The relative mRNA level of MoAP1 in the mycelium from the lesions of (A) generated by asiR1115-treated Guy11 and control. The expression of MoAP1 was determined by qRT-PCR. The relative MoAP1 mRNA levels were measured by using the mRNA level of M. oryzae MoAP1. Values are means ± standard deviations (SD) of three replicates. Asterisks (∗∗) above the bars indicate significant differences at P < 0.01 as determined by student’s t-test. All experiments repeated three times with similar results.