Figure 3.
HIF3A promoter CpG islands were highly methylated in gestational diabetes mellitus patients. (a) BSP analysis of CpG islands methylation status in HIF3A promoter region: Two CpG islands, from −290 to −158 (CpG island 1) and from −123 to +64 (CpG island 2) in HIF3A promoter region (the transcriptional start site was set as +1), were predicted via online software “methprimer” (Top); BSP analysis of the promoter CpG islands methylation status in the omental tissue from healthy control or gestational diabetes mellitus patients were shown in the bottom. (b) Pearson correlation analysis of HIF3A expression and CpG islands methylation degree; HIF3A mRNA level was significantly negatively correlated with the CpG islands methylation degree (R 2 = 0.8422, p < 0.001)