Figure 2.

The effect of novel mutations on potentially altering the protein stability. (a) Western blotting assay was performed to detect the expression level of mutated PTK7 constructs with indicated antibodies. Relative expression level which normalized to GAPDH as control, was quantified by Image J. Error bars represent ±SD for triplicate experiments. *p < 0.05, Student's t test was performed to the wildtype. (b) The protein expression levels of R630S PTK7 and wildtype construct are examined by immunocytochemistry. (c) HEK293T cells were transfected with both R630S and wildtype constructs then treated with CHX at the 1 µg/ml concentrations for 24 hr for blocking protein translation. Error bars represent ±SD for triplicate experiments, GAPDH as the control. *p < 0.05 Student's t test was performed to the treated and untreated. Scale bar: 20 µm