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. 2019 Jan 28;7(4):e00584. doi: 10.1002/mgg3.584

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© 2019 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of PTK7 variants on the interaction with Dvl2 and Vangl2. (a) Coimmunoprecipitation assay detecting PTK7‐Dvl2 binding in HEK293T cells by transiently co‐expression of HA‐tagged Dvl2 and tGFP‐tagged PTK7 (wildtype and variants) constructs. (b) Coimmunoprecipitation assay detecting PTK7‐Vangl2 binding in HEK293T cells by transiently co‐expression of HA‐tagged Vangl2 and tGFP‐tagged PTK7 (wildtype and variants) constructs. IP: immunoprecipitation; WCL: whole cell lysate; con: negative control