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. 2019 Jan 28;7(4):e00584. doi: 10.1002/mgg3.584

Figure 3.

Figure 3

The effect of PTK7 variants on the interaction with Dvl2 and Vangl2. (a) Coimmunoprecipitation assay detecting PTK7‐Dvl2 binding in HEK293T cells by transiently co‐expression of HA‐tagged Dvl2 and tGFP‐tagged PTK7 (wildtype and variants) constructs. (b) Coimmunoprecipitation assay detecting PTK7‐Vangl2 binding in HEK293T cells by transiently co‐expression of HA‐tagged Vangl2 and tGFP‐tagged PTK7 (wildtype and variants) constructs. IP: immunoprecipitation; WCL: whole cell lysate; con: negative control