Table 3.
Most frequent and abundant OTUs (genus level) found in each inocula group at the beginning of the experiment and after 11 days of enrichment as determined by a SIMPER test.
| OTU | Identity | Sim/SD | Contr% | OTU | Identity | Sim/SD | Contr% |
|---|---|---|---|---|---|---|---|
| Rumen day 0 | Rumen 11 days | ||||||
| Average similarity: 83.62 | Average similarity: 84.48 | ||||||
| Cumulative similarity: 74.75 |
Cumulative similarity: 71.35 |
||||||
| 13 | Ruminococcus | 17.95 | 11.78 | 2 | Pseudomonas | 21.74 | 31.92 |
| 14 | Millionella | 8.08 | 10.37 | 4 | Aeromonas | 11.06 | 20.66 |
| 19 | Lutispora | 20 | 9.63 | 12 | Carnobacterium | 44.07 | 6.9 |
| 23 | Prevotella | 17.35 | 8.61 | 10 | Enterococcus | 8.7 | 6.37 |
| 20 | Succiniclasticum | 21.75 | 8.61 | 5 | Leuconostoc | 7.05 | 5.5 |
| 34 | Holdemania | 17.7 | 7.14 | – | – | – | – |
| 41 | Victivallis | 8.73 | 6.7 | – | – | – | – |
| 37 | Spiroplasma | 7.95 | 6.36 | – | – | – | – |
| 65 | Paraprevotella | 6.58 | 5.57 | – | – | – | – |
| Sludge day 0 | Sludge 11 days | ||||||
| Average similarity: 86.29 | Average similarity: 88.04 | ||||||
| Cumulative similarity: 70.65 |
Cumulative similarity: 72.44 |
||||||
| 1 | Pseudomonas | 23.69 | 31.02 | 3 | Clostridium | 38.07 | 21.85 |
| 7 | Lentimicrobium | 12.37 | 17.7 | 9 | Pseudomonas | 35.25 | 15.7 |
| 6 | Proteiniphilum | 21.67 | 11.11 | 5 | Leuconostoc | 41.32 | 14.01 |
| 16 | Clostridium | 19.89 | 5.61 | 1 | Pseudomonas | 7.5 | 10.81 |
| 8 | Acinetobacter | 3.53 | 5.21 | 11 | Macellibacteroides | 10.62 | 10.06 |
| R + S day 0 | R + S 11 days | ||||||
| Average similarity: 77.03 | Average similarity: 81.93 | ||||||
| Cumulative similarity: 71.67 |
Cumulative similarity: 71.89 |
||||||
| 1 | Pseudomonas | 3.53 | 17.82 | 3 | Clostridium | 57.48 | 14.66 |
| 6 | Proteiniphilum | 4.22 | 12.51 | 10 | Enterococcus | 146.71 | 11.62 |
| 13 | Ruminococcus | 34.51 | 7.68 | 8 | Acinetobacter | 52.91 | 9.55 |
| 7 | Lentimicrobium | 5.91 | 7.58 | 12 | Carnobacterium | 12.88 | 8.91 |
| 21 | Leptolinea | 9.82 | 4.93 | 1 | Pseudomonas | 14.91 | 7.1 |
| 15 | Paludibacter | 50.93 | 4.84 | 2 | Pseudomonas | 3.48 | 6.85 |
| 14 | Millionella | 10.88 | 4.68 | 5 | Leuconostoc | 2.37 | 6.67 |
| 24 | Mucinivorans | 65.25 | 4.68 | 4 | Aeromonas | 18.41 | 6.53 |
| 16 | Clostridium | 6.36 | 3.84 | – | – | – | – |
| 11 | Macellibacteroides | 2.35 | 3.12 | – | – | – | – |
Contr%, an OTU’s individual contribution to the average composition of each inoculum/sampling day group in descending order. Sim/SD, the ratio of the average contribution divided by the standard deviation of those contributions across all sample pairs in each group (Sim/SD). This metric allows the identification of OTUs that contributed consistently to the average bacterial composition of each group of replicates.