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. 2018 Dec 22;7(1):2. doi: 10.3390/vaccines7010002

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multistep growth kinetics of CHIKV-NoLS in C6/36 cells after extended in vitro passage. C6/36 cells were infected with CHIKV-WT, P0 CHIKV-NoLS or P5 CHIKV-NoLS at an MOI of 0.1 PFU/cell. Supernatants were collected at the indicated time points, and infectious virus was quantified by plaque assay. **, p < 0.01, and ***, p < 0.001. Statistical analyses were performed using two-way ANOVA with Bonferroni post-tests. Each symbol represents the mean ± standard error from 3 independent experiments.