Table 2.
Formulations of experimental diets.
| Phase 1 | Phase 2 | Phase 3 | |||||
|---|---|---|---|---|---|---|---|
| Days on Feed | 0–7 | 7–21 | 21–42 | ||||
| Ingredient (%) | Control | 1.25% FO 1 | 3.12% AL 2 | Control | 1.25% FO 1 | 3.12% AL 2 | Common |
| Soybean meal | 30.00 | 30.00 | 30.00 | 35.00 | 35.00 | 35.00 | 30.00 |
| Corn (NRC 3; 8.3%CP 4) | 45.28 | 45.28 | 43.42 | 41.00 | 41.00 | 39.14 | 48.82 |
| Wheat | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 |
| Monocalcium phosphate | 1.52 | 1.52 | 1.52 | 1.24 | 1.24 | 1.24 | 0.97 |
| Limestone | 1.30 | 1.30 | 1.30 | 1.25 | 1.25 | 1.25 | 1.17 |
| Salt | 0.50 | 0.50 | 0.50 | 0.40 | 0.40 | 0.40 | 0.40 |
| Mineral/Vitamin premix 5 | 0.60 | 0.60 | 0.60 | 0.60 | 0.60 | 0.60 | 0.60 |
| L-Lysine | 0.37 | 0.37 | 0.37 | 0.26 | 0.26 | 0.26 | 0.35 |
| L-Methionine | 0.15 | 0.15 | 0.15 | 0.11 | 0.11 | 0.11 | 0.09 |
| L-Threonine | 0.24 | 0.24 | 0.24 | 0.12 | 0.12 | 0.12 | 0.10 |
| L-Tryptophan | 0.02 | 0.02 | 0.02 | – | – | – | – |
| Vitamin E | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | – |
| Microalgae 6 | – | – | 3.12 | – | – | 3.12 | – |
| Fish oil | – | 1.25 | – | – | 1.25 | – | – |
| Corn oil | 5.00 | 3.75 | 3.75 | 5.00 | 3.75 | 3.75 | 2.50 |
| Calculated nutrient composition 7 | |||||||
| Metabolizable energy, kcal/Kg | 3472 | 3471 | 3472 | 3479 | 3478 | 3479 | 3358 |
| Net energy, kcal/Kg | 2612 | 2611 | 2636 | 2592 | 2591 | 2602 | 2509 |
| CP (%) | 20.85 | 20.85 | 20.69 | 22.65 | 22.65 | 22.5 | 20.97 |
| Crude fat (ether extract, %) | 7.06 | 6.96 | 7.42 | 6.99 | 6.89 | 7.35 | 4.81 |
| SID 8 Lysine (%) | 1.21 | 1.21 | 1.21 | 1.25 | 1.25 | 1.25 | 1.2 |
| SID Methionine + cysteine (%) | 0.71 | 0.71 | 0.71 | 0.72 | 0.72 | 0.71 | 0.66 |
| SID Threonine (%) | 0.86 | 0.86 | 0.86 | 0.81 | 0.81 | 0.81 | 0.73 |
| SID Tryptophan (%) | 0.24 | 0.24 | 0.24 | 0.25 | 0.25 | 0.25 | 0.23 |
| Analzed nutrient composition (%) | |||||||
| Dry matter | 92.27 | 92.04 | 92.11 | 91.87 | 92.55 | 92.23 | na |
| CP | 17.70 | 18.53 | 20.31 | 22.02 | 21.62 | 22.61 | na |
| Crude fat | 5.22 | 5.69 | 7.02 | 6.11 | 6.96 | 7.05 | na |
| Calcium | 0.82 | 0.96 | 0.88 | 0.81 | 0.86 | 0.88 | na |
| Phosphorus | 0.74 | 0.76 | 0.73 | 0.69 | 0.74 | 0.73 | na |
| Sodium | 0.18 | 0.20 | 0.19 | 0.15 | 0.18 | 0.16 | na |
| Potassium | 0.86 | 0.88 | 0.92 | 1.02 | 0.98 | 1.05 | na |
| Magnesium | 0.17 | 0.18 | 0.18 | 0.19 | 0.19 | 0.20 | na |
1 FO, Fish oil; 2 AL, algae; 3 NRC, National Research Council 2012; 4 CP, crude protein; 5 supplied per kg of diet: vitamin A, 12,000 IU as retinyl acetate; vitamin D3, 1,200 IU as cholecalciferol; vitamin E, 48 IU as DL-α-tocopherol acetate; vitamin K, 3 mg as menadione; vitamin B12, 0.03 mg; pantothenic acid, 18 mg; riboflavin, 6 mg; choline, 600 mg; folic acid, 2.4 mg; niacin, 30 mg; thiamine, 18 mg; pyridoxine, 1.8 mg; biotin, 200 µg; Cu, 18 mg as CuSO4·5H2O; Fe, 120 mg as FeSO4; Mn, 24 mg as MnSO4; Zn, 126 mg as ZnO; Se, 0.36 mg as FeSeO3; I, 0.6 mg as KI; DSM, Ayr, ON, Canada; 6 microalgae supplied by Alltech Inc., and supplied as a dried biomass, containing 15.8% CP, 70% crude fat and 17% DHA; 7 calculated on the basis of the NRC (2012) ingredient values; 8 SID, standardized ileal digestible.