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. 2019 Mar 20;11(3):282. doi: 10.3390/v11030282

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of the V672F substitution on the growth of PEDV in VeroE6-APN cells. (A) Schematic representation of infectious clones used for generation of recombinant PEDV bearing S_G2 or S_G2-V672F. (B) VeroE6-APN cells were adsorbed with supernatants harvested from HEK293T cells transfected with the infectious clone of rgPEDV-AVCT12mCherry-S_G2-V672F. The expression of mCherry was assessed by fluorescence microscopy at indicated times after adsorption. Scale 50 µm. (C) Growth kinetics of recombinant PEDV bearing S_G2, S_G2-V672F, S_YN144, and S_YN144 with F672V substitution in VeroE6-APN and Huh-7 cells. Error bars represent mean ± SD. * p < 0.05.