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. 2019 Mar 4;11(3):218. doi: 10.3390/v11030218

Figure 2.

Figure 2

Construction and in vitro characterization of B2c-3G, a recombinant RABV expressing three copies of G. (A) Schematic diagram of B2c recombinant virus carrying two extra G genes, each with its own reading frame. The arrows indicate the location of restriction enzyme sites in the genome of RABV. (B) Growth curves of B2c-3G and B2c were generated in NA and BSR cells. (C) Morphological analysis and length of B2c-3G and B2c were compared by electron microscopy after negative staining. (D) ELISA of G content in RABVs at 103, 104, and 105 FFU/ml. (E) Western blot analysis of viral protein in NA cells and viral protein incorporated into virions. The ratio between G and N was calculated from the intensity of the band using Image-Pro Plus 6.0. The significance of differences between viral titers or OD450 of B2c-3G and B2c were assessed by the unpaired t-test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.