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. 2019 Mar 4;11(3):218. doi: 10.3390/v11030218

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Removal of G from RABV virions after treatment with DTT/NP40 or subtilisin. (A) Morphological analysis of DTT/NP40- or subtilisin-treated RABV by electron microscopy after negative staining. (B) RABV-G protein on virions was detected by staining with RABV-G-specific antibodies labeled with 5-nm immunogold particles. (C) Western blot analysis of viral N and G proteins incorporated into virions. (D) Viral genomic RNA from RABV treated with or without DTT/NP40 or subtilisin and analyzed by qRT-PCR. (E) Growth curves of B2c-3G and B2c in the NA and BSR cells.