Figure 2.

Endolysin engineering strategy. The modular structures of endolysins are shown; catalytic domains (enzymatically active domain (EAD)) of endolysin A and endolysin B (green and white rectangles) are originally connected to their binding domains (cell wall-binding domain (CDB)) (grey and red rectangles) via cognate linkers (straight and wavy lines). An engineered chimeric endolysin can be created by domain shuffling using the endolysin A EAD and the endolysin B CBD with an appropriate linker.