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. 2019 Apr 18;19(2):137–150. doi: 10.3727/105221618X15372014086197

Figure 6.

Figure 6

Blocking IL-6 signaling in mice reduces tumor formation in DEN–alcohol model of liver tumorigenesis. Male PRMT1 flox/flox (WT) and PRMT1 flox/flox LysM Cre (MKO) littermates received a single injection of 10 mg/kg of DEN at 2 weeks of age. At 3 months of age, mice were fed Lieber–DeCarli alcohol liquid diet (4.8% alcohol) for 7 weeks. Starting at week 3, mice received weekly injections of soluble gp130 protein. n = 6 mice per group. (A) Diagram shows the experiment protocol. (B) Surface tumors number. *p < 0.05; **p < 0.01; ns: p > 0.05. (C) H&E staining of liver sections showing normal area and steatosis area. Scale bars: 100 μm. (D) Left: liver/body weight ratios. Right: average TG content in male mice on alcohol untreated or treated with sgp130. *p < 0.05; **p < 0.01. (E) Immunohistochemistry staining for Mrc1 (CD206) and pSTAT3. Scale bars: 100 μm. Bottom: Relative staining intensity. n = 4 mice per group. **p < 0.01; ns: p > 0.05. (F) Relative liver mRNA. *p < 0.05; **p < 0.01; ns: p > 0.05.