Figure 7.

Sex differences in treatment response. The treatment response to vitamin D analogs was assessed separately in male and female mice. Animals were randomized to receive SD alone (control), or SD supplemented with either calcitriol or EB for 4 weeks, starting at 3 weeks after hydrodynamic transfection. Each group had four to six animals. Tumor growth was monitored using (a) Gaussia luciferase (g-luc) activity or (b) AFP assayed at 3, 6, 9, or 12 weeks after transfection Data are presented as mean value ± SEM at 9 weeks. Survival curves were estimated, in three to four animals per group, by Kaplan–Meier analysis and compared by log-rank test (c) in male mice (p = 0.13), and (d) in female mice (p = 0.21).