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Transformation efficiency of BSRF1 KO mutant virus. Viruses in culture medium of HEK293 EBV-BAC cells were prepared and the titers were determined using Akata(−) cells. After normalization of the titers, 10-fold serial dilutions were prepared and used to separately infect peripheral blood mononuclear cells (PBMCs) from two healthy donors (ex1 and ex2). Bars indicate 50% TU/mL values. Cell-clone numbers are also shown (#3, #4).
