Figure 7.
Incorporation of BSRF1 and BALF1 in virions. EBV particles were prepared from B95-8 cell culture medium and transfected with the indicated expression vectors. The medium was precleared via low-speed centrifugation and filtration. Virions were collected by ultracentrifugation in the absence (lane 1) or presence of NP-40 (lane 2) or NP-40 plus 300 mM NaCl (lane 3), followed by immunoblotting with the indicated antibodies.