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. 2019 Mar 23;8(1):19. doi: 10.3390/biology8010019

Figure 1.

Figure 1

Stromal-derived factor 1 (SDF-1) expression is decreased in epididymal adipose tissue (eWAT) from OsmrFKO mice. Gene expression was analyzed in (a) eWAT from chow-fed OsmrFKO or floxed Osmr (control) mice (n = 6–8/genotype). (b) Osmr knockdown confirmation in eWAT from high fat diet-fed mice (n = 6–8/genotype). (c) Fractionated eWAT from high-fat-diet-fed OsmrFKO mice (n = 2–3 pooled samples/genotype containing tissue from 2–3 mice per sample) and expression levels were normalized to Ppia. (d) Protein levels of various cytokines and chemokines were assessed in eWAT from OsmrFKO and control mice fed a high-fat diet (n = 6/genotype). Black bars = control (floxed) animals; gray bars = OsmrFKO. * p < 0.05, **** p < 0.001 vs. floxed controls.