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Expression of HuNoV VP1 by LAB vector. LAB-VP1 strain was grown to mid-log phase (O.D. = 0.4) in M17 medium and centrifuged. The cell pellets and supernatants were collected. Proteins from supernatants were precipitated using trichloroacetic acid (TCA) and pelleted by centrifugation. The total protein (10 μg) from both the supernatant and cell pellet was analyzed by (A) SDS and PAGE and (B) Western blot using HuNoV VP1-specific antibody.
