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. 2019 Mar 14;11(3):259. doi: 10.3390/v11030259

Figure 2.

Figure 2

Western blot analysis of anti-filovirus mAbs against wild-type filovirus NP. 10 µg of purified irradiated EBOV (A), SUDV (B), RESTV (C), TAFV (D), and MARV (E) were resolved in SDS-PAGE gels, and the proteins were transferred into nitrocellulose membranes. The membranes were treated with (A) 15D10 and 6E5; (B) 3F12, 11G9, 6G5, and 12F12; (C) 2A10 and 7E7; (D) 4B10, 2A10, 8G9, and 1H3; (E) 3E10, 6E9, 5E9, 8H4, 7A12, and 1G10; mAbs. Fluorescent-labeled goat anti-mouse monoclonal secondary antibodies detected the NP. 10 µg of purified RESTV, TAFV, SUDV, EBOV, BDBV, and MARV were resolved into two separate SDS-PAGE gels, the resolved proteins were transferred into nitrocellulose membrane, and the blots were treated with 2G10 (F) and 4F9 (G) mAbs to detect different filovirus NPs.