Figure 3.

Sera from immunized pigs enhances ASFV infection in vitro. ASFV strain Ba71V pre-incubated with either pre-immune sera (black bars) as a control, or immune sera (grey bars) collected at 49 days post vaccination was used to infect Vero cells. After 16 hours post infection the percentage of p72 expression was determined by FACS using a specific monoclonal antibody (17LD3) to measure virus infection. Mean with standard deviations are shown for each vaccinated group of pigs. Significance was determined by student t test (***; p-value < 0.001). The results represent biological triplicates and duplicates, for Groups 1 (left) and 2 (right), respectively.