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. 2019 Feb 15;7(1):21. doi: 10.3390/vaccines7010021

Figure 4.

Figure 4

Stability of pre-fusion F conformation in VLPs. Equivalent amounts of F protein in VLPs were incubated for one hour at the temperatures, pH, or salt concentrations indicated. The binding of mAb D25 to the VLPs was quantified using anti-human IgG coupled to HRP. Results are presented as mAb 25 bound at each condition as a percent of the binding at 4 °C (panel A), or pH 7.5 (panel B), or 0.15 M salt (Panel C). Results are the mean with standard deviations of three separate determinations. Differences were not statistically significant (student t test).