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(a) Photo of a bolt with a helical minichannel. (b) Photo of the bolt connected to a nut. (c,d) Working principle of nut and bolt microfluidics. Finger-pricked whole blood (red) fills the space inside the nut (yellow). By rotating the bolt (blue) in the clockwise direction, the blood is introduced to the helical minichannel through a hole made at the end of the bolt.
